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KMID : 0613820130230070886
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Journal of Life Science
2013 Volume.23 No. 7 p.886 ~ p.892
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Ethanol Production from Glycerol using Pachysolen tannophilus in a Surface-aerated Fermentor
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Kim Yi-Ok
Choi Woon-Yong
Kang Do-Hyung
Lee Hyeon-Yong
Jung Kyung-Hwan
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Abstract
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We investigated ethanol production from glycerol after screening of the yeast Pachysolen tannophilus ATCC 32691. For yeast to produce ethanol form glycerol, it is important that aeration is finely controlled. Therefore, we attempted to produce ethanol using a surface-aerated fermentor. When 880 ml of YPG medium (1% yeast extract, 2% peptone, 2% glycerol) was used to produce ethanol, the optimal aeration conditions for ethanol production were a surface aeration rate and agitation speed of 500 ml/min and 300 rpm, respectively. In a fed-batch culture, the maximum ethanol production and the maximum ethanol yield from glycerol (Ye/g) was 5.74 g/l and 0.166, respectively, after 90 hr using the surface-aerated fermentor.
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KEYWORD
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Ethanol, fed-batch culture, glycerol, Pachysolen tannophilus, surface aeration
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